Capillary electrophoresis with wavelength-resolved laser-induced fluorescence detection

被引：0
|
作者：
Xin Zhang
Jeffrey N. Stuart
Jonathan V. Sweedler
机构：
[1] Department of Chemistry,
[2] University of Illinois,undefined
[3] Urbana IL 61801,undefined
来源：
Analytical and Bioanalytical Chemistry | 2002年 / 373卷
关键词：
Review Capillary electrophoresis Laser-induced fluorescence Wavelength-resolved fluorescence;
D O I：
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中图分类号：
学科分类号：
摘要：
Capillary electrophoresis (CE) enables rapid separations with high separation efficiency and compatibility with small sample volumes. Laser-induced fluorescence detection can result in extremely low limits of detection in CE. Single-channel fluorescence detection, however, furnishes little qualitative information about a species being detected, except for its CE migration time. Use of multidimensional information often enables unambiguous identification of analytes. Combination of CE with information-rich wavelength-resolved fluorescence detection is analogous with ultraviolet–visible diode-array detection and furnishes both qualitative and quantitative chemical information about target species. This review discusses recent advances in wavelength-resolved laser-induced fluorescence detection coupled with CE, with an emphasis on instrument design.
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页码：332 / 343
页数：11
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