Transmembrane carbonic anhydrase isozymes IX and XII in the female mouse reproductive organs

被引：25
|
作者：
Hynninen P. [1 ]
Hämäläinen J.M. [2 ]
Pastorekova S. [3 ]
Pastorek J. [3 ]
Waheed A. [4 ]
Sly W.S. [4 ]
Tomas E. [1 ]
Kirkinen P. [1 ]
Parkkila S. [2 ]
机构：
[1] Dept. of Gynecology and Obstetrics, University of Tampere, Tampere University Hospital, Tampere
[2] Institute of Medical Technology, University of Tampere, Tampere University Hospital, Tampere
[3] Center of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Bratislava
[4] E. A. Doisy Dept. of Biochem./Molec., Saint Louis Univ. School of Medicine, St. Louis, MO
来源：
Reproductive Biology and Endocrinology | / 2卷 / 1期
关键词：
Carbonic Anhydrase; Sperm Motility; Normal Rabbit Serum; Carbonic Anhydrase Activity; Human Endometrium;
D O I：
10.1186/1477-7827-2-73
中图分类号：
学科分类号：
摘要：
Background: Carbonic anhydrase (CA) classically catalyses the reversible hydration of dissolved CO2 to form bicarbonate ions and protons. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. Methods: The present study was designed to investigate the expression of transmembrane CAs, CA IX and XII, in the mouse uterus, ovary and placenta. The expression of CA IX and XII was examined by immunoperoxidase staining method and western blotting. CA II and XIII served as positive controls since they are known to be present in the mouse reproductive tract. Results: The data of our study indicated that CA XII is expressed in the mouse endometrium. Only very faint signal was observed in the corpus luteum of the ovary and the placenta remained mainly negative. CA IX showed weak reaction in the endometrial epithelium, while it was completely absent in the ovary and placenta. Conclusion: The conservation of CA XII expression in both mouse and human endometrium suggests a role for this isozyme in reproductive physiology. © 2004 Hynninen et al; licensee BioMed Central Ltd.
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页数：10
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