Concanavalin A electrochemical sensor based on the surface blocking principle at an ion-selective polymeric membrane

被引：0
|
作者：
Marcin Pawlak
Günter Mistlberger
Eric Bakker
机构：
[1] University of Geneva,Department of Inorganic and Analytical Chemistry
[2] Graz University of Technology,Department of Analytical and Food Chemistry
[3] Université de Genève,Department of Inorganic and Analytical Chemistry, Sciences II
来源：
Microchimica Acta | 2015年 / 182卷
关键词：
Biosensor; Concanavalin A; Click chemistry; Ion selective electrodes; Chronopotentiometry;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We report on a new electrochemical sensor for Concanavalin A. It is based on blocking the surface of plasticized PVC membranes that were covalently modified with D-mannose using click chemistry. The interaction of D-mannose with Concanavalin A on the surface perturbs the flux of a marker ion for which the ion-selective membrane is responsive, and this results in a change in the electrochemical signal. The sensor was characterized using a variety of electrochemical protocols, and results were confirmed by quartz crystal microbalance experiments. The lowest limit of detection (10 μg mL−1) was obtained using a membrane containing a cation exchanger and tetrabutylammonium ion as the marker ion.
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页码：129 / 137
页数：8
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