Dynabeads protein G antibody conjugates combined with modified brain heart infusion broth for the enrichment and separation of Bacillus cereus in artificially contaminated vegetables

被引：0

作者：

Shuai Wei

Fereidoun Forghani

Youn-Seo Park

Byung-Jae Park

Kun-Ho Seo

Deog-Hwan Oh

机构：

[1] The University of Tennessee Health Science Center,Department of Microbiology, Immunology and Biochemistry

[2] Konkuk University,KU Center for Food Safety, College of Veterinary Medicine

[3] Kangwon National University,Department of Food Science and Biotechnology, School of Bioconvergence Science and Technology

来源：

Food Science and Biotechnology | 2016年 / 25卷

关键词：

immunomagnetic separation; Dynabeads® protein G; enrichment;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A modified brain heart infusion (MBHI) broth and a protocol of immunomagnetic separation (IMS) using antibody-coated Dynabeads® protein G were developed for the enrichment and separation of Bacillus cereus in artificially contaminated vegetable samples. The MBHI consisted of BHI and 0.34 g/L magnesium sulfate, 12.08 g/L sodium pyruvate, 1.82 g/L yeast extract, and polymyxin B. The amount of immunomagnetic beads (IMBs) and immunoreaction time were optimized. The capture efficiency was 58.32% with 0.4 mg IMBs when the immunoreaction time was 20 min. Capture of B. cereus by IMBs did not interfere with competing flora. Pre-enrichment IMS was validated with four B. cereus strains in artificially contaminated baby sprouts, bean sprouts, lettuce, and spinach at two levels (∼0.1 and ∼1 CFU/g). We were able to detect and isolate B. cereus in 40/40 samples of vegetables contaminated at 0.1 CFU/g with IMS after 6 h of enrichment in MBHI.

引用

页码：941 / 947

页数：6