Optimization of Callus Culture and Extraction Method for Elevated Production of Phenolic Antioxidants from Jasminum malabaricum Wight.

Jasminum malabaricum Wight. (Family: Oleaceae) a climber, is endemic to Western Ghats of India. Rapid proliferating callus cultures were established from leaf explants using varying concentrations of auxins with cytokinins. Culture medium consisting of MS basal supplemented with 2, 4-D (9.05 μM) showed good proliferation and addition of cytokinins (BA 11.09 μM and/or TDZ 2.27 μM) resulted in retarded proliferation. Optimization of phenolic-antioxidants from different parts of J. malabaricum showed continuous shaking method to be productive over microwave assisted method. The optimized method involves less input and good yield of phenolic-antioxidants. Obtained callus showed good antioxidant activity (DPPH and FRAP assays) in comparison with leaves and stem extracts and also had higher total phenolics. The optimized protocol for callus culture extraction revealed that continuous shaking extraction is cost-effective and suitable for elevated production of phenolic-antioxidants from callus cultures of J. malabaricum.