Winogradskyella undariae sp. nov., a member of the family Flavobacteriaceae isolated from a brown algae reservoir

A novel bacterial strain, designated WS-MY5T, capable of degrading a variety of polysaccharides was isolated from a brown algae (Undaria pinnatifida) reservoir at Wando in the South Sea, South Korea. Strain WS-MY5T was found to grow optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WS-MY5T falls within the clade comprising Winogradskyella species, clustering with the type strains of Winogradskyellapacifica, Winogradskyellaarenosi, Winogradskyellarapida and Winogradskyellathalassocola, with which it exhibited 16S rRNA gene sequence similarity values of 97.3–98.8 %. It exhibited sequence similarity values of 93.0–96.2 % to the type strains of the other recognized Winogradskyella species. Strain WS-MY5T was found to contain MK-6 as the predominant menaquinone and anteiso-C15:0, iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH and iso-C15:1 G as the major fatty acids. The major polar lipids of strain WS-MY5T were identified as phosphatidylethanolamine, two unidentified lipids and two unidentified aminolipids. The DNA G+C content of strain WS-MY5T was determined to be 33.2 mol% and its DNA–DNA relatedness values with the type strains of W. pacifica, W. arenosi, W. rapida and W. thalassocola were in the range 16–28 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, enabled strain WS-MY5T to be differentiated from the recognized Winogradskyella species. On the basis of the data presented here, strain WS-MY5T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella undariae sp. nov. is proposed. The type strain is WS-MY5T (=KCTC 32261T=CCUG 63832T).