N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

被引：0
|
作者：
Y. -H. Noh
H. -S. Cho
D. -H. Kim
O. H. Kim
J. Park
S. -A. Lee
H. -S. Yang
D. -S. Sohn
W. Kim
D. Kim
Y. H. Chung
K. Y. Kim
S. S. Kim
W. B. Lee
机构：
[1] Chung-Ang University,Department of Cell Biology and Anatomy, College of Medicine
[2] Chung-Ang University,Department of Otorhinolaryngology
[3] Chung-Ang University,Head and Neck Surgery, College of Medicine
[4] Chung-Ang University,Department of Thoracic Surgery, College of Medicine
来源：
Molecular Biology | 2012年 / 46卷
关键词：
Akt; N-cadherin; -acetylcysteine; P19 embryonic stem cell; differentiation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.
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页码：664 / 669
页数：5
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