Production of exopolysaccharide by Pseudomonas sp. ATCC 31461 (Pseudomonas elodea ) using whey as fermentation substrate

被引:0
|
作者
A. M. Dlamini
P. S. Peiris
机构
[1] School of Applied and Environmental Science,
[2] University of Western Sydney–Hawkesbury,undefined
[3] Bourke Street,undefined
[4] Richmond,undefined
[5] NSW 2753,undefined
[6] Australia. Fax: 61 2 045 701 267,undefined
来源
Applied Microbiology and Biotechnology | 1997年 / 47卷
关键词
Enzyme; Viscosity; Fermentation; Pseudomonas; Lactose;
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摘要
An improved strain of Pseudomonas sp. ATCC 31461 (Pseudomonas elodea), capable of producing broth viscosities of 11 000 and 4700 mPa s (cP) when grown in enriched whey permeate and enriched sweet whey broths respectively, was isolated. The isolation was by serial transfers of the parent on lactose-rich and sweet whey broths. Maximum viscosities and biopolymer production were observed in 25% (v/v) whey concentration. In whey concentrations of 50% (v/v) or greater, residual glucose was detected in the broth and biopolymer production was low. This strain is capable of totally utilising the lactose in up to 50% (v/v) whey in 64 h. Enzyme activities suggest that the transport of lactose in P. elodea is by the permease system as opposed to the phosphotransferase system. The location of β-galactosidase is mainly intracellular. The improved strain is able to utilise lactose better than the parent and produce 1.6 times more intracellular β-galactosidase activity compared to the parent.
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页码:52 / 57
页数:5
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