Recent developments in FEI's in situ cryo-electron tomography workflow

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|
作者：
Alexander Rigort
机构：
[1] FEI Munich GmbH,
来源：
Nature Methods | 2016年 / 13卷
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摘要：
Studying the molecular machinery of cells from atomic details to the cellular context and beyond is a great challenge for cell biology. It requires the integration of dynamic and structural information obtained using different ranges of spatial resolution. In order for this to be accomplished, a comprehensive workflow is needed covering vitreous freezing, cryo-fluorescence microscopy, sample thinning by focused ion beam microscopy, and high-resolution cryo-electron tomography. This Application Note provides an overview of FEI's integrated product suite, providing a complete cryo-transmission electron microscopy (cryo-TEM) tomography workflow.
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页码：iii / iv
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