The cytotoxicity of PM2.5 and its effect on the secretome of normal human bronchial epithelial cells

被引:0
|
作者
Zhigang Sui
Xiaoyao Song
Yujie Wu
Rui Hou
Jianhui Liu
Baofeng Zhao
Zhen Liang
Jiping Chen
Lihua Zhang
Yukui Zhang
机构
[1] CAS Key Laboratory of Separation Science for Analytical Chemistry,
[2] National Chromatographic R. & A. Center,undefined
[3] Dalian Institute of Chemical Physics,undefined
[4] Chinese Academy of Science,undefined
[5] Environmental Assessment and Analysis Group,undefined
[6] Key Laboratory of Separation Science for Analytical Chemistry,undefined
[7] Dalian Institute of Chemical Physics,undefined
[8] Chinese Academy of Sciences,undefined
[9] Zhang Dayu School of Chemistry,undefined
[10] Dalian University of Technology,undefined
来源
Environmental Science and Pollution Research | 2022年 / 29卷
关键词
Secretome; Proteomic analysis; PM; Cytotoxicity; Apoptosis; Extracellular matrix;
D O I
暂无
中图分类号
学科分类号
摘要
Exposure to airborne fine particulate matter (PM2.5) induced various adverse health effects, such as metabolic syndrome, systemic inflammation, and respiratory disease. Many works have studied the effects of PM2.5 exposure on cells through intracellular proteomics analyses. However, changes of the extracellular proteome under PM2.5 exposure and its correlation with PM2.5-induced cytotoxicity still remain unclear. Herein, the cytotoxicity of PM2.5 on normal human bronchial epithelia cells (BEAS-2B cells) was evaluated, and the secretome profile of BEAS-2B cells before and after PM2.5 exposure was investigated. A total of 83 proteins (58 upregulated and 25 downregulated) were differentially expressed in extracellular space after PM2.5 treatment. Notably, we found that PM2.5 promoted the release of several pro-apoptotic factors and induced dysregulated secretion of extracellular matrix (ECM) constituents, showing that the abnormal extracellular environment attributed to PM2.5-induced cell damage. This study provided a secretome data for the deep understanding of the molecular mechanism underlying PM2.5-caused human bronchial epithelia cell damage.
引用
收藏
页码:75966 / 75977
页数:11
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