A new pregnane hexaglycoside from Adonis multiflora

A new pregnane hexaglycoside, named amurensioside L (1), was isolated from the whole plant of Adonismultiflora. Amurensioside L (1) emerged to be new pregnane hexaglycoside which we fully spectroscopically characterized. The chemical structure of compound 1 was established as 14β,20R-epoxy-3β-hydroxypregna-4,6-dien-18-oic acid-20-lactone 3-O-β-d-glucopyranosyl-(1 → 4)-O-β-d-diginopyranosyl-(1 → 4)-O-β-d-cymaropyranosyl-(1 → 4)-O-β-d-cymaropyranosyl-(1 → 4)-O-β-d-oleandropyranosyl-(1 → 4)-O-β-d-oleandropyranoside on the basis of spectroscopic analyses including nuclear magnetic resonance spectrometry, high-resolution fast atom bombardment mass spectrometry, and infrared spectroscopy.