Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans

被引：0
|
作者：
Hideto Takami
Yuichi Nogi
Koki Horikoshi
机构：
[1] Deep-sea Microorganisms Research Group,
[2] Japan Marine Science and Technology Center,undefined
[3] 2-15 Natsushima,undefined
[4] Yokosuka 237-0061,undefined
[5] Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp,undefined
来源：
Extremophiles | 1999年 / 3卷
关键词：
Key words Alkaliphilic Bacillus halodurans AH-101; DNA–DNA hybridization; Phylogenetic tree; Identification;
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摘要：
Alkaliphilic Bacillus sp. AH-101 was characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA–DNA hybridization analyses were performed. Phylogenetic analysis of strain AH-101 based on comparison of 16S rDNA sequences revealed that this strain is closely related to Bacillus halodurans. DNA–DNA hybridization of AH-101 and related Bacillus reference strains showed that the highest level of DNA–DNA relatedness (88%) was found between strain AH-101 and the B. halodurans type strain (DSM497). Our findings demonstrate that strain AH-101 is a member of the species B. halodurans.
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页码：293 / 296
页数：3
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