Separation of supercoiled and open-circular plasmid DNA by liquid-liquid counter-current chromatography

被引：0
|
作者：
D. Kendall
A.J. Booth
M.S. Levy
G.J. Lye
机构：
[1] University College London,The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
Chromatography; HPLC; Organic Chemistry; Mobile Phase; Fractionation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We report for the first time the use of liquid-liquid counter-current chromatography (CCC) for the preparative scale fractionation of plasmid DNA. Almost complete fractionation of supercoiled and open circular plasmid DNA (6.9 kb) could be achieved using a phase system comprising 12.5% (w/w) PEG 600 and 18% (w/w) K2HPO4. Experiments were carried out on a Brunel J-type CCC machine (100 ml PTFE coil) at a mobile phase flow rate of 0.5 ml min− 1 and a rotational speed of 600 rpm. Compared to conventional HPLC techniques the capacity of CCC is not limited by the surface area of resin available for adsorption.
引用
收藏
页码：613 / 619
页数：6
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