Preparation and characterization of a bovine serum albumin-catalase conjugate

被引:0
|
作者
Tao Hu
Zhiguo Su
机构
[1] Chinese Academy of Sciences,National Laboratory of Biochemical Engineering, Institute of Process Engineering
来源
Biotechnology Letters | 2002年 / 24卷
关键词
bovine serum albumin; catalase; O; free radicals; -succinimidyl 3-(2-pyridyldithio)propionate;
D O I
暂无
中图分类号
学科分类号
摘要
A bovine serum albumin-catalase conjugate was prepared using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The purified conjugate had an Mr of 380 kDa. The half-life time of the conjugate was 6, 69 and 65 min after heat treatment, urea treatment and trypsin hydrolysis, respectively, whereas that of native catalase was 3, 22, 28 min, respectively.
引用
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页码:1027 / 1030
页数:3
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