New polymeric nanosorbents for selective binding of biological macromolecules

Acrylamide-based hydrogel nanoparticles, which had hydrodynamic radii in aqueous dispersions of about 100-180 nm, were prepared by miniemulsion polymerization. The redox initiation at the interfacial boundary made it possible to conduct synthesis at an ambient temperature with a high rate and resulted in latexes of enhanced aggregative stability. The original method for nanoparticle functionalization by aminophenylboronic acid was elaborated for their application as affinity sorbents for 1,2-diols. The ability to reversibly bind ribonucleoside (inosine) and glycated protein (hemoglobin) has been demonstrated. The binding capacity relative to inosine and glycated hemoglobin was determined as a function of the phenylboronate group content. © 2014 Pleiades Publishing, Ltd.