LC Separation of Fatty Acid Ceramides Using a Two Column System

被引：0
|
作者：
Monika Budvytiene
Nataliya Babenko
Jolanta Liesiene
机构：
[1] Kaunas University of Technology,Institute of Biology
[2] Kharkov Karazin National University,undefined
来源：
Chromatographia | 2009年 / 69卷
关键词：
Column liquid chromatography; Two column system; Coated stationary phases; -Stearoyl sphingosine and ; -palmitoyl sphingosine; Ceramides;
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学科分类号：
摘要：
Type II ceramides were separated according to the length of their fatty acid alkyl chain using two column system. The packing of the first column was non-polar adsorbent prepared by coating of macroporous spherical silica with cationized poly(vinyl alcohol) of low substitution degree. Commercial normal phase column Lichrosorb Si 60 was used as a second column in this system. Elution was performed in an isocratic mode using methanol:chloroform 50:50 (v/v) as an eluent.
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页码：1473 / 1475
页数：2
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