Acute toxicity assays using Danio rerio and Daphnia magna to assess hot-spring drainage in the Shibukuro and Tama Rivers (Akita, Japan)

We investigated the lethal toxicity of Shibukuro and Tama river water near the inflow of Tamagawa hot-spring water in Akita Prefecture, Japan. We first measured metal concentrations in both rivers. We detected iron, arsenic, and aluminum; the concentrations of each tended to decrease from upstream to downstream. We next examined the influence of river water on zebrafish Danio rerio and water flea Daphnia magna. We observed lethal effects in both species, with Daphnia magna more sensitive to toxicity than Danio rerio. For both species, the toxic effects of river water decreased with increasing distance downstream from the inflow of hot-spring water. Our results show that the metals discharged from Tamagawa hot spring have a negative effect on aquatic organisms.