Expression of neutralizing epitope of porcine epidemic diarrhea virus in potato plants

被引：0
|
作者：
Young-Sook Kim
Tae-Jin Kang
Yong-Suk Jang
Moon-Sik Yang
机构：
[1] Chonbuk National University,Division of Biological Sciences and the Institute for Molecular Biology and Genetics
[2] Chonbuk National University,Institute of Basic Science
[3] Chonbuk National University,Division of Biological Sciences and the Research Center for Bioactive Materials
来源：
Plant Cell, Tissue and Organ Culture | 2005年 / 82卷
关键词：
neutralizing epitope; plant expression; porcine epidemic; porcine epidemic diarrhea virus; transgenic potato;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. A neutralizing epitope of porcine epidemic diarrhea virus (PEDV) gene containing SEKDEL was expressed in potato using Agrobacterium-mediated transformation system. Putative transgenic plants were regenerated, and genomic PCR confirmed the presence of PEDV epitope gene in the potato plants. Based on the ELISA results, epitope of PEDV protein made up approximately 0.1% of the total soluble tuber protein.
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页码：125 / 130
页数：5
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