Genotyping of recombinant Pichia pastoris strains

被引：0
|
作者：
José M. Viader-Salvadó
Eddy L. Cab-Barrera
Luis J. Galán-Wong
Martha Guerrero-Olazarán
机构：
[1] Universidad Autónoma de Nuevo León. Ave. Pedro de Alba s/n,Instituto de Biotecnología, Facultad de Ciencias Biológicas
来源：
Cellular & Molecular Biology Letters | 2006年 / 11卷
关键词：
Pichia pastoris; Amplified-fragment length polymorphism (AFLP); Clonal variation;
D O I：
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摘要：
A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.
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页码：348 / 359
页数：11
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