Abstract: The prospects of interplanetary missions make it relevant to develop standard tests to find criteria for the correction of preventive measures in a long space flight in order to ensure a peak in physical performance by the time the interplanetary activity is completed. Currently, tests are being developed to assess physical performance. One of them can be the currently developed locomotor test with a stepwise increasing load in the active mode of movement of the treadmill. It is of interest to assess the effect of this load on changes in the profile of proteins associated with the cardiovascular system from the standpoint of the possibility of using them as markers of its response to physical activity. In article analyzes the results of an experimental study of the proteome of human urine after a dosed step-increasing load and discusses the possible role of the identified proteins that can be attributed to the functioning of the cardiovascular system. The study involved practically healthy volunteers aged 18.6 ± 0.7 years, weighing 75.7 ± 8 kg (n = 12). The urine proteome was evaluated by chromatography-mass spectrometry and analyzed by bioinformatics methods. For the first time, data on the proteomic response to a locomotor test with a stepwise increasing load in the active mode of web movement in a group homogeneous in autonomic status are presented. As a result of the analysis, 429 proteins were identified, 69 of which significantly changed. Based on bioinformatics analysis, processes related to the work of the heart, vascular tone, and vascular permeability were identified. Ten proteins are described that are associated with the processes of the quick response of the cardiovascular system to dosed physical activity. The obtained results will help in choosing standard criteria for assessing the physiological cost of physical activity. © 2021, Pleiades Publishing, Inc.
