Production of medium-chain-length polyhydroxyalkanoates by pseudomonas aeruginosa with fatty acids and alternative carbon sources

In this study, medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were produced by Pseudomonas aeruginosa using different carbon sources. Decanoic acid induced the highest (9.71% [±0.7]) mcl-PHAs accumulation in bacterial cells at 47 h. The cells preferred to accumulate and degrade the poly-hydroxyoctanoate than polyhydroxydecanoate (PHD) during early stage and final stage, of the growth, respectively. The production cost of mcl-PHAs can be reduced by using edible oils as the carbon source. The bacteria accumulated 6% (±0.7) of mcl-PHAs in the presence of olive oil. Besides, reused oil was another potential carbon source for the reduction of the production cost of mcl-PHAs. Overall, PHD was the major constitutent in the accumulated mcl-PHAs.