Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

被引：0
|
作者：
Takakazu Nakabayashi
Hui-Ping Wang
Masataka Kinjo
Nobuhiro Ohta
机构：
[1] Hokkaido University,Research Institute for Electronic Science (RIES)
[2] Zhejiang University,Department of Physiology, School of Medicine
[3] Hokkaido University,Faculty of Advanced Life Science
来源：
Photochemical & Photobiological Sciences | 2008年 / 7卷
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摘要：
We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.
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页码：668 / 670
页数：2
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