We investigated four stream types in four different bioregions, classified by catchment area and altitude, and stressed by different degrees of organic pollution and habitat alteration. We examined a macro-invertebrate based multimetric approach for Austrian rivers as a potential assessment method within the European Water Framework Directive. Benthic macro-invertebrate data (100 samples including reference sites) were used to develop a multimetric index for each stream type and targeted stressors. Sites were pre-classified based on physical, chemical, and land use criteria into five ecological quality classes. More than 200 biological metrics were tested for their sensitivity to the targeted stressors, their spatial and temporal variability and their ability to discriminate between different types and degrees of stress. Metrics for index development were selected to reflect different levels of information including ecosystem, community, and individual levels (Karr, 1991; Barbour et al., 1995; Gerritsen, 1995). Combinations of metrics were selected to distinguish best between non or slightly impaired and stressed sites (evaluated by calculating discrimination efficiency values and power analysis). The resulting four indices comprised seven to nine metrics from five to seven metric categories, and distinguished reference/slightly disturbed sites from stressed sites with close to 100% efficiency. The indices can form the basis for stressor-specific assessment of stream condition.