Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants

被引：0

作者：

David B. Kastner

Viktor Kharazia

Rhino Nevers

Clay Smyth

Daniela A. Astudillo-Maya

Greer M. Williams

Zhounan Yang

Cristofer M. Holobetz

Luca Della Santina

Dilworth Y. Parkinson

Loren M. Frank

机构：

[1] University of California,Department of Psychiatry and Behavioral Sciences

[2] University of California,Kavli Institute for Fundamental Neuroscience and Department of Physiology

[3] University of California,Deparment of Ophthalmology

[4] University of California,Bakar Computational Health Science Unit

[5] Lawrence Berkeley National Labs,Advanced Light Source

[6] Howard Hughes Medical Institute,undefined

来源：

Scientific Reports | / 10卷

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摘要：

Anatomic evaluation is an important aspect of many studies in neuroscience; however, it often lacks information about the three-dimensional structure of the brain. Micro-CT imaging provides an excellent, nondestructive, method for the evaluation of brain structure, but current applications to neurophysiological or lesion studies require removal of the skull as well as hazardous chemicals, dehydration, or embedding, limiting their scalability and utility. Here we present a protocol using eosin in combination with bone decalcification to enhance contrast in the tissue and then employ monochromatic and propagation phase-contrast micro-CT imaging to enable the imaging of brain structure with the preservation of the surrounding skull. Instead of relying on descriptive, time-consuming, or subjective methods, we develop simple quantitative analyses to map the locations of recording electrodes and to characterize the presence and extent of hippocampal brain lesions.

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