A ratiometric fluorescent nanoprobe consisting of ssDNA-templated silver nanoclusters for detection of histidine/cysteine, and the construction of combinatorial logic circuits

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作者
Xiaodong Lin
Zhe Hao
Haotian Wu
Minyang Zhao
Xia Gao
Shuo Wang
Yaqing Liu
机构
[1] Tianjin University of Science and Technology,State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety (Ministry of Education), College of Food Engineering and Biotechnology
[2] Nankai University,Tianjin Key Laboratory of Food Science and Health, School of medicine
[3] Beijing Technology and Business University,Beijing Advanced Innovation Center for Food Nutrition and Human Health
来源
Microchimica Acta | 2019年 / 186卷
关键词
Ratiometric nanoprobe; Amino acid; DNA-AgNCs; Fluorometric biosensor; Logic gates;
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摘要
A ratiometric fluorescent nanoprobe consisting of ssDNA-templated silver nanoclusters (DNA-AgNCs) as dual fluorophore has been constructed for highly sensitive and selective detection of cystein (Cys) and histidine (His). The DNA-AgNCs displays dual emission in that photoexcitation at 470 nm results in weak-green fluorescence peaking at 560 nm, while excitation at 550 nm gives strong red fluorescence with a peak at 595 nm. It is found that copper ions (Cu2+) enhance the green fluorescence but quenche the red fluorescence. A ratiometric nanoprobe for Cys (or His) is designed that is based on the competitive interaction of Cys (or His), Cu2+ and DNA-AgNCs. Cys can be distinguished from His by adding Ni2+ as the masking agent, andr His can be distinguished from Cys by adding N-ethylmaleimide (NEM) as the masking agent, respectively. The limits of detection are 5.1 and 4.5 nM for Cys and His, respectively. Furthermore, the ratiometric fluorescent nanoprobe is used for constructing combinatorial logic circuits in parallel, including OR//NOR and INHIBIT//IMPLICATION (INH//IMP).
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