Solvent Exchange Rates of Side-chain Amide Protons in Proteins

被引：0
|
作者：
Ponni Rajagopal
Bryan E. Jones
Rachel E. Klevit
机构：
[1] University of Washington,Department of Biochemistry
来源：
Journal of Biomolecular NMR | 1998年 / 11卷
关键词：
HPr; side-chain amide proton; solvent exchange rates; temperature coefficients;
D O I：
暂无
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学科分类号：
摘要：
Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values > 8. An NOE between a side-chain amide proton and a bound water molecule was also observed.
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页码：205 / 212
页数：7
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