A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy

被引：0
|
作者：
Richard N Day
Wen Tao
Kenneth W Dunn
机构：
[1] Indiana University School of Medicine,Department of Cellular and Integrative Physiology
[2] Indiana,Department of Medicine, Division of Nephrology
[3] Indiana University Medical Center,undefined
[4] Indiana,undefined
来源：
Nature Protocols | 2016年 / 11卷
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摘要：
This protocol describes a simple pipeline for imaging FRET biosensor probes with two-photon laser scanning microscopy (TPLSM). Validated FRET standards are described that can be used with any TPLSM setup to ensure sensitive detection of FRET ratios.
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页码：2066 / 2080
页数：14
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