Validation of the Method of Identification and Quantitative Determination of Bacitracin in the Form of Bacitracin Derivative with Dabsyl Chloride by Thin-Layer Chromatography and Densitometry

被引：0
|
作者：
Jan Krzek
Joanna Piotrowska
Barbara Żuromska-Witek
Urszula Hubicka
Ewelina Łysoń
机构：
[1] Medical College of Jagiellonian University,Department of Inorganic and Analytical Chemistry
来源：
JPC – Journal of Planar Chromatography – Modern TLC | 2013年 / 26卷
关键词：
Pharmaceutical research; Bacitracin; Dabsyl chloride; TLC; Densitometry;
D O I：
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学科分类号：
摘要：
A thin-layer chromatographic (TLC)-densitometric method has been developed for identification and quantitative determination of bacitracin derivative with dabsyl chloride. The analysis of antibiotic was achieved on the silica gel TLC plates with fluorescent indicator with n-butanol-2-butanone-25% ammonia-water (10:5:2:2, v/v) as mobile phase. The densitometric measurements were made at 460 nm. Under these conditions, good separation of chosen polypeptide antibiotic from reagent used to make a complex was obtained.
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页码：67 / 72
页数：5
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