Evaluation of a Recombinant Antigen Enzyme-Linked Immunosorbent Assay (ELISA) in the Diagnostics of Antinuclear Antibodies (ANA) in Children with Rheumatic Disorders

被引：0
|
作者：
A. Reiff
H. Haubruck
M. D. Amos
机构：
[1] Keck School of Medicine,
[2] University of Southern California School of Medicine,undefined
[3] Childrens’ Hospital Los Angeles,undefined
[4] Los Angeles,undefined
[5] California,undefined
[6] USA,undefined
[7] DIARECT AG,undefined
[8] Freiburg,undefined
[9] Germany,undefined
[10] Life Technologies Inc.,undefined
[11] Rockville,undefined
[12] Maryland,undefined
[13] USA,undefined
来源：
Clinical Rheumatology | 2002年 / 21卷
关键词：
Key words:ANA – Children – ELISA – IFA – Rheumatology;
D O I：
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学科分类号：
摘要：
The reliability and accuracy of ELISAs for the detection of circulating ANA in children with rheumatic diseases has recently been questioned. In this study we evaluated an allegedly superior ELISA method using recombinant antigens in a paediatric population with various rheumatic conditions and compared it to a conventional Hep-2 IFA assay. Sera from 123 children (204 blood samples) were simultaneously tested by conventional ANA immunofluorescence on Hep-2 cells (ANA-IFA) and recombinant antigen ELISA (rELISA). There were 44 children with systemic lupus erythematosus (SLE), 29 with juvenile rheumatoid arthritis (JRA), eight with mixed connective tissue disease (MCTD), eight with reactive arthritis, five with juvenile fibromyalgia syndrome, three with dermatomyositis (JDMS) and 31 with other diagnoses.
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页码：103 / 107
页数：4
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