MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis
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作者:
Pengfei Xu
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机构:The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
Pengfei Xu
Jun Chang
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机构:The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
Jun Chang
Guangwen Ma
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机构:The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
Guangwen Ma
Faxue Liao
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机构:The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
Faxue Liao
Tangbing Xu
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机构:The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
Tangbing Xu
Yunfeng Wu
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机构:The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
Yunfeng Wu
Zongsheng Yin
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机构:The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
Zongsheng Yin
机构:
[1] The First Affiliated Hospital of Anhui Medical University,Department of Orthopaedics
[2] Anhui Public Health Clinical Center,Department of Orthopaedics
来源:
BMC Musculoskeletal Disorders
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23卷
关键词:
Steroid-induced osteonecrosis of femoral head;
microRNA-145;
Bone marrow stromal mesenchymal stem cells;
Proliferation;
Differentiation;
GABARAPL1;
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摘要:
Steroid-induced osteonecrosis of femoral head (SANFH) involves impaired differentiation of bone marrow mesenchymal stem cells (BMSC), the mechanism of which is regulated by multiple microRNAs. Studies have shown that miR-145 is a key regulatory molecule of BMSC cells, but its mechanism in steroid-induced femur head necrosis remains unclear. The present study mainly explored the specific mechanism of miR-145 involved in SANFH. In this study dexamethasone, a typical glucocorticoid, was used to induce osteogenic differentiation of BMSC cells. Western blot, qPCR, CCK8 and flow cytometry were used to investigate the effects of miR-145 on the proliferation and differentiation of BMSC. The relationship between miR-145 and GABA Type A Receptor Associated Protein Like 1(GABARAPL1) was identified using dual luciferase reports and the effects of the two molecules on BMSC were investigated in vitro. The results showed that miR-145 was up-regulated in SANFH patients, while GABARAPL1 was down-regulated. Inhibition of miR-145 can improve apoptosis and promote proliferation and activation of BMSC. GABARAPL1 is a downstream target gene of miR-145 and is negatively regulated by miR-145. In conclusion, miR-145 regulates the proliferation and differentiation of glucocorticoid-induced BMSC cells through GABARAPL1 and pharmacologically inhibit targeting miR-145 may provide new aspect for the treatment of SANFH.
机构:
Chinese Acad Med Sci, Dept Plast Surg 4, Plast Surg Hosp, Peking Union Med Coll, Beijing 100144, Peoples R ChinaChinese Acad Med Sci, Dept Plast Surg 4, Plast Surg Hosp, Peking Union Med Coll, Beijing 100144, Peoples R China
Wang, Bingqing
Yu, Peng
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Chinese Acad Med Sci, Dept Orthopaed Surg, Peking Union Med Coll Hosp, Peking Union Med Coll, Beijing 100730, Peoples R ChinaChinese Acad Med Sci, Dept Plast Surg 4, Plast Surg Hosp, Peking Union Med Coll, Beijing 100144, Peoples R China
Yu, Peng
Li, Tao
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机构:
Ocean Univ Qingdao, Affiliated Hosp, Dept Orthopaed Surg, Qingdao 266003, Peoples R ChinaChinese Acad Med Sci, Dept Plast Surg 4, Plast Surg Hosp, Peking Union Med Coll, Beijing 100144, Peoples R China
Li, Tao
Bian, Yanyan
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机构:
Chinese Acad Med Sci, Dept Orthopaed Surg, Peking Union Med Coll Hosp, Peking Union Med Coll, Beijing 100730, Peoples R ChinaChinese Acad Med Sci, Dept Plast Surg 4, Plast Surg Hosp, Peking Union Med Coll, Beijing 100144, Peoples R China
Bian, Yanyan
Weng, Xisheng
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机构:
Chinese Acad Med Sci, Dept Orthopaed Surg, Peking Union Med Coll Hosp, Peking Union Med Coll, Beijing 100730, Peoples R ChinaChinese Acad Med Sci, Dept Plast Surg 4, Plast Surg Hosp, Peking Union Med Coll, Beijing 100144, Peoples R China