Effect of Interleukin-1β on the Expression of Tight Junction Proteins in the Culture of HaCaT Keratinocytes

We studied the effect of IL-1β on the expression of tight junction proteins (occludin and claudins) in cultured HaCaT keratinocytes and changes of transepithelial resistance. Addition of IL-1β had little effect on transepithelial resistance, increased the expression of claudin-1, and did not modify the expression of occludin. In other tissues, IL-1β also increases claudin-1 expression, but significantly decreases occludin expression. These changes are accompanied by the reduction of transepithelial resistance. The IL-1β-induced increase in the expression of claudin-1 in cultured HaCaT keratinocytes simulates the appearance of claudin-1 at the early stage of skin wound healing. It is accompanied by an increase in IL-1β concentration in the wound fluid.