Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR

被引：0

作者：

Cristina Grúas

Silvia Llambi

M. Victoria Arruga

机构：

[1] University of Zaragoza,Laboratory of Cytogenetics and Molecular Genetics, Faculty of Veterinary

[2] UDELAR,Departamento de Genética y Mejora Animal, Instituto de Producción Animal, Facultad de Veterinaria

来源：

Archives of Microbiology | 2014年 / 196卷

关键词：

culture method; Real-time PCR; .;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127–128, 2002; Doleans et al. in J Clin Microbiol 42:458–460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459–466, 2008). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene.

引用

页码：63 / 71

页数：8

共 50 条

[1] Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR
Gruas, Cristina
Llambi, Silvia
Victoria Arruga, M.
ARCHIVES OF MICROBIOLOGY, 2014, 196 (01) : 63 - 71
[2] Detection of Legionella pneumophila in water samples by species-specific real-time and nested PCR assays
Fiume, L
Bucca Sabattini, MA
Poda, G
LETTERS IN APPLIED MICROBIOLOGY, 2005, 41 (06) : 470 - 475
[3] Specific Real-Time PCR for Simultaneous Detection and Identification of Legionella pneumophila Serogroup 1 in Water and Clinical Samples
Merault, N.
Rusniok, C.
Jarraud, S.
Gomez-Valero, L.
Cazalet, C.
Marin, M.
Brachet, E.
Aegerter, P.
Gaillard, J. L.
Etienne, J.
Herrmann, J. L.
Lawrence, C.
Buchrieser, C.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (05) : 1708 - 1717
[4] Legionella detection from water samples by real-time PCR
Franzin, Laura
Cabodi, Daniela
Bonfrate, Nicoletta
LEGIONELLA: STATE OF THE ART 30 YEARS AFTER ITS RECOGNITION, 2006, : 446 - +
[5] Assessment of real-time PCR for quantification of Legionella spp. in spa water
Guillemet, T. A.
Levesque, B.
Gauvin, D.
Brousseau, N.
Giroux, J. -P.
Cantin, P.
LETTERS IN APPLIED MICROBIOLOGY, 2010, 51 (06) : 639 - 644
[6] Usefulness of real-time PCR as a complementary tool to the monitoring of Legionella spp. and Legionella pneumophila by culture in industrial cooling systems
Touron-Bodilis, A.
Pougnard, C.
Frenkiel-Lebosse, H.
Hallier-Soulier, S.
JOURNAL OF APPLIED MICROBIOLOGY, 2011, 111 (02) : 499 - 510
[7] Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems
Yaradou, Diaraf Farba
Hallier-Soulier, Sylvie
Moreau, Sophie
Poty, Florence
Hillion, Yves
Reyrolle, Monique
Andre, Janine
Festoc, Gabriel
Delabre, Karine
Vandenesch, Francois
Etienne, Jerome
Jarraud, Sophie
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (05) : 1452 - 1456
[8] Fast detection and quantification of Legionella spp. and Legionella pneumophila
Pennanec, X.
Dufour, A.
Charreteur, C.
Haras, D.
Rehel, K.
MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (08) : 28 - 28
[9] Detection of Legionella pneumophila by real-time PCR for the mip gene
Wilson, DA
Yen-Lieberman, B
Reischl, U
Gordon, SM
Procop, GW
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) : 3327 - 3330
[10] Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples
Diederen, Bram M. W.
de Jong, Caroline M. A.
Marmouk, Faical
Kluytmans, Jan A. J. W.
Peeters, Marcel F.
Van der Zee, Anneke
JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 56 (01) : 94 - 101

← 1 2 3 4 5 →