cDNA cloning, structural organization, and expression of the sheep NRAMP1 gene

被引：0

作者：

Vincent Bussmann

Isabelle Lantier

Frédérique Pitel

Sylvie Patri

François Nau

Philippe Gros

Jean-Michel Elsen

Frédéric Lantier

机构：

[1] Pathologie Infectieuse et Immunologie,

[2] INRA de Tours-Nouzilly,undefined

[3] 37380 Nouzilly,undefined

[4] France,undefined

[5] Génétique Cellulaire,undefined

[6] INRA de Toulouse,undefined

[7] 31326 Castanet-Tolosan,undefined

[8] France,undefined

[9] Laboratoire de Génétique Cellulaire et Moléculaire,undefined

[10] CHU de Poitiers,undefined

[11] 86000 Poitiers,undefined

[12] France,undefined

[13] Laboratoire d'Immunologie Moléculaire,undefined

[14] IBMIG,undefined

[15] CNRS ESA 6031,undefined

[16] 86000 Poitiers,undefined

[17] France,undefined

[18] Department of Biochemistry,undefined

[19] McGill University,undefined

[20] Montreal H3G 1Y6,undefined

[21] Canada,undefined

[22] Station d'Amélioration Génétique des Animaux Domestiques,undefined

[23] INRA de Toulouse,undefined

[24] 31326 Castanet-Tolosan,undefined

[25] France,undefined

来源：

Mammalian Genome | 1998年 / 9卷

关键词：

cDNA Library; cDNA Cloning; Transcription Start; Domestic Animal; Genomic Organization;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Mouse resistance to several intracellular pathogens including Mycobacteria, Leishmania, and Salmonella is under the control of the Chromosome (Chr) 1 Natural Resistance Associated Macrophage Protein I gene (Nramp1). This gene could have an economic and health importance for domestic animals and humans as well. Therefore, equivalents of the NRAMP1 gene have been cloned by several research groups in various animal species. To study in sheep the influence of the NRAMP1 gene on the susceptibility to intracellular pathogens induced diseases, we have cloned the sheep NRAMP1 cDNA by screening a splenic cDNA library. The genomic organization of the sheep NRAMP1 gene was then determined by sequencing the exon/intron boundaries. The transcription start points (tsp) from the NRAMP1 mRNA have been located with primer extension experiments. RT-PCR reactions have been used to determine the profile of mRNA expression of this gene.

引用

页码：1027 / 1031

页数：4

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