Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast

被引：0
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作者：
E. V. Trubnikova
E. P. Isakova
Yu. I. Deryabina
A. V. Nikolaev
机构：
[1] Kursk State University,Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
[2] Russian Academy of Sciences,Schmidt Joint Institute of Physics of the Earth
[3] Russian Academy of Sciences,undefined
来源：
Doklady Biochemistry and Biophysics | 2016年 / 468卷
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摘要：
A modified variant of the β-galactosidase gene from Escherichia coli (lacZ) has been obtained. This was designed for building transcriptional reporter constructs, the product of which possesses a higher proteolytic resistance in the Yarrowia lipolytica yeasts. The study of the activity of mitochondrial voltagedependent porin (voltage-dependent anion channel, VDAC) showed a high efficiency of the reporter system based on the designed lacZ reporter compared to the wild-type lacZ gene.
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页码：190 / 192
页数：2
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