Counting colonies of clonogenic assays by using densitometric software

被引：0
|
作者：
Maximilian Niyazi
Ismat Niyazi
Claus Belka
机构：
[1] CCC Tübingen,Department of Radiation Oncology
[2] Bureau for Technique and Documentation,undefined
来源：
Radiation Oncology | / 2卷
关键词：
Gray Level; Routine Activity; Interindividual Difference; Clonogenic Assay; Microscopical Evaluation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features.
引用
收藏
相关论文
共 50 条
  • [1] Counting colonies of clonogenic assays by using densitometric software
    Niyazi, Maximilian
    Niyazi, Ismat
    Belka, Claus
    RADIATION ONCOLOGY, 2007, 2 (1)
  • [2] Counting clonogenic assays from normoxic and anoxic irradiation experiments manually or by using densitometric software
    Wouters, An
    Pauwels, Bea
    Lambrechts, Hilde A. J.
    Pattyn, Greet G. O.
    Ides, Johan
    Baay, Marc
    Meijnders, Paul
    Lardon, Filip
    Vermorken, Jan B.
    PHYSICS IN MEDICINE AND BIOLOGY, 2010, 55 (07): : N167 - N178
  • [3] Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: Comparison with manual counting
    Cai, Zhongli
    Chattopadhyay, Niladri
    Liu, Wenchao Jessica
    Chan, Conrad
    Pignol, Jean-Philippe
    Reilly, Raymond M.
    INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2011, 87 (11) : 1135 - 1146
  • [4] An improved method for staining cell colonies in clonogenic assays
    Guda, Kishore
    Natale, Leanna
    Markowitz, Sanford D.
    CYTOTECHNOLOGY, 2007, 54 (02) : 85 - 88
  • [5] An improved method for staining cell colonies in clonogenic assays
    Kishore Guda
    Leanna Natale
    Sanford D. Markowitz
    Cytotechnology, 2007, 54 : 85 - 88
  • [6] A reliable automated counting method to assess clonogenic assays.
    Keyhani, A
    Pearlman, M
    Pagliaro, LC
    Liu, B
    Jendiroba, DB
    Kantarjian, H
    Freireich, EJ
    BLOOD, 1998, 92 (10) : 311B - 311B
  • [7] DETECTION OF LEUKEMIA USING CLONOGENIC ASSAYS
    LOWENBERG, B
    DELWEL, R
    INTERNATIONAL JOURNAL OF CELL CLONING, 1985, 3 (04): : 263 - 264
  • [8] CLONOGENIC CLUSTERS IN CLONOGENIC ASSAYS FOR PREDICTION OF CHEMOTHERAPY SENSITIVITY
    TISMAN, G
    HSU, MYK
    CLINICAL RESEARCH, 1982, 30 (01): : A70 - A70
  • [9] CLONOGENIC AND NON-CLONOGENIC INVITRO CHEMOSENSITIVITY ASSAYS
    WEISENTHAL, LM
    LIPPMAN, ME
    CANCER TREATMENT REPORTS, 1985, 69 (06): : 615 - 632
  • [10] Relevance of clonogenic assays in hematotoxicology
    Parent-Massin, D
    CELL BIOLOGY AND TOXICOLOGY, 2001, 17 (02) : 87 - 94
12345