Piezoelectric immunosensor for the direct and rapid detection ofFrancisella tularensis

被引：0
|
作者：
M. Pohanka
P. Skládal
机构：
[1] Center of Biological Defense,Department of Biochemistry, Faculty of Science
[2] Masaryk University,undefined
来源：
Folia Microbiologica | 2007年 / 52卷
关键词：
Quartz Crystal Microbalance; Tularemia; Francisella Tularensis; Mouse Polyclonal Antibody; Quartz Crystal Resonator;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agentFrancisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detectF. tularensis with the limit of detection 105 CFU/mL with a typical measuring cycle >5 min. The sensor was successfully evaluated for rapid detection ofF. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
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页码：325 / 330
页数：5
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