Fabrication of amperometric cholesterol biosensor based on SnO2 nanoparticles and Nafion-modified carbon paste electrode

被引：0
|
作者：
Gözde Aydoğdu Tığ
Derya Koyuncu Zeybek
Şule Pekyardımcı
机构：
[1] Ankara University,Department of Chemistry, Faculty of Science
[2] Dumlupinar University,Department of Biochemistry, Faculty of Science and Arts
来源：
Chemical Papers | 2016年 / 70卷
关键词：
cholesterol biosensor; carbon paste electrode; amperometry; SnO; nanoparticles;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study reports the fabrication of an amperometric cholesterol biosensor based on cholesterol oxidase (ChOx), SnO2NPs and Nafion-modified carbon paste enzyme electrodes (CPE/SnO2NPs-ChOx/Naf). The electrochemical characterisations of BCPE and CPE/SnO2NPs were performed using CV and EIS. The determination of cholesterol was carried out by electrochemical oxidation of H2O2 at 0.6 V vs. Ag/AgCl. The CPE/SnO2NPs-ChOx/Naf presented a linear range from 0.20 μ.mol L−1 to 4.95 μmol L−1 with a low limit of detection (0.04 μ.mol L−1). In addition, the optimal values for pH and temperature were found to be 7.5 and 35°C, respectively. The CPE/SnO2NPs-ChOx/Naf was used for the determination of cholesterol in serum samples and good results were obtained.
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页码：695 / 705
页数：10
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