Enzyme kinetic characterization of microbe-produced urease for microbe-driven calcite mineralization

被引:0
|
作者
Li Li
Chun-xiang Qian
Yong-hao Zhao
Yun-tian Zhu
机构
[1] Nanjing University of Science and Technology,Nano
[2] Southeast University,structural Materials Center, School of Materials Science and Engineering
[3] North Carolina State University,Green Construction Materials Research Institution, School of Materials Science and Engineering
来源
Reaction Kinetics, Mechanisms and Catalysis | 2013年 / 108卷
关键词
Cementation; Michaelis–Menten equation; Microbe calcite precipitation; Urea hydrolysis; Urease-producing bacteria;
D O I
暂无
中图分类号
学科分类号
摘要
Just as calcite precipitation rate is equal to CO32− formation rate, this CO32− formation rate is equal to that of urea hydrolysis in microbial calcite precipitation. This formative rate of CO32− evolved a mode to assay the urea hydrolysis rate, which was influenced by urea and Ca2+ concentrations, pH, and temperature. To contrive the highest Vmax of the Michaelis–Menten enzyme kinetics equation for the fastest urea hydrolysis rate, 35 °C, pH 10, 2 M urea and 2 M Ca2+ were the optimal conditions based on the studies.
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页码:51 / 57
页数:6
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