Cloning and Sequencing of the Gene of Site-Specific Nickase N.BspD6I

被引：0
|
作者：
T. A. Perevyazova
E. A. Rogulin
L. A. Zheleznaya
N. I. Matvienko
机构：
[1] Institute of Theoretical and Experimental Biophysics,Russian Academy of Sciences
[2] Institute of Protein Research,Russian Academy of Sciences
来源：
Biochemistry (Moscow) | 2003年 / 68卷
关键词：
site-specific endonuclease; site-specific nickase; cloning; sequencing;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A fragment of chromosomal DNA from Bacillus species D6 containing the gene of nickase N.BspD6I and the regions adjacent to its 5"- and 3"-ends was cloned and sequenced. The nucleotide sequence of the nickase gene, except of one neutral change, is homologous to the nicking endonuclease N.BstNBI gene sequenced by Higgens et al. (2001). After integration of a PCR-copy of the nickase gene into an expression vector pET28b under the control of the phage T7 promoter, specific nicking activity was detected in the lysates of transformed E. coli cells.
引用
收藏
页码：984 / 987
页数：3
相关论文
共 50 条
  • [1] Cloning and sequencing of the gene of site-specific nickase N.BspD6I
    Perevyazova, T.A.
    Rogulin, E.A.
    Zheleznaya, L.A.
    Matvienko, N.I.
    Biokhimiya, 2003, 68 (09): : 1203 - 1207
  • [2] Cloning and sequencing of the gene of site-specific nickase N.BspD6I
    Perevyazova, TA
    Rogulin, EA
    Zheleznaya, LA
    Matvienko, NI
    BIOCHEMISTRY-MOSCOW, 2003, 68 (09) : 984 - 987
  • [3] Plasmid pRARE as a vector for cloning to construct a superproducer of the site-specific nickase N.BspD6I
    Rogulin, EA
    Perevyazova, TA
    Zheleznaya, LA
    Matvienko, NI
    BIOCHEMISTRY-MOSCOW, 2004, 69 (10) : 1123 - 1127
  • [4] Plasmid pRARE as a Vector for Cloning to Construct a Superproducer of the Site-Specific Nickase N.BspD6I
    E. A. Rogulin
    T. A. Perevyazova
    L. A. Zheleznaya
    N. I. Matvienko
    Biochemistry (Moscow), 2004, 69 : 1123 - 1127
  • [5] Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I
    Kachalova, GS
    Rogulin, EA
    Artyukh, RI
    Perevyazova, TA
    Zheleznaya, LA
    Matvienko, NI
    Bartunik, HD
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2005, 61 : 332 - 334
  • [6] Some properties of site-specific nickase BspD6I and the possibility of its use in hybridization analysis of DNA
    Zheleznaya, LA
    Perevyazova, TA
    Zheleznyakova, EN
    Matvienko, NI
    BIOCHEMISTRY-MOSCOW, 2002, 67 (04) : 498 - 502
  • [7] Some properties of site-specific nickase BspD6I and the possibility of its use in hybridization analysis of DNA
    Inst. of Theor. Experim. Biophys., RAS, Pushchino, Moscow Region, 142290, Russia
    Biokhimiya, 2002, 67 (04): : 595 - 600
  • [8] Some Properties of Site-Specific Nickase BspD6I and the Possibility of Its Use in Hybridization Analysis of DNA
    L. A. Zheleznaya
    T. A. Perevyazova
    E. N. Zheleznyakova
    N. I. Matvienko
    Biochemistry (Moscow), 2002, 67 : 498 - 502
  • [9] Oligomerization of site-specific nicking endonuclease BspD6I at high protein concentrations
    S. A. Sekerina
    A. V. Grishin
    A. Yu. Ryazanova
    R. I. Artyukh
    E. A. Rogulin
    A. K. Yunusova
    T. S. Oretskaya
    L. A. Zheleznaya
    E. A. Kubareva
    Russian Journal of Bioorganic Chemistry, 2012, 38 : 376 - 382
  • [10] Oligomerization of site-specific nicking endonuclease BspD6I at high protein concentrations
    Sekerina, S. A.
    Grishin, A. V.
    Ryazanova, A. Yu.
    Artyukh, R. I.
    Rogulin, E. A.
    Yunusova, A. K.
    Oretskaya, T. S.
    Zheleznaya, L. A.
    Kubareva, E. A.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2012, 38 (04) : 376 - 382
12345