Purification and characterisation of NAD+-dependent xylitol dehydrogenase from Fusarium oxysporum

被引：0
|
作者：
G. Panagiotou
D. Kekos
B.J. Macris
P. Christakopoulos
机构：
[1] National Technical University of Athens,Biotechnology Laboratory, Department of Chemical Engineering
来源：
Biotechnology Letters | 2002年 / 24卷
关键词：
purification; xylitol dehydrogenase;
D O I：
暂无
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学科分类号：
摘要：
An NAD+-dependent xylitol dehydrogenase (XDH) from Fusarium oxysporum, a key enzyme in the conversion of xylose to ethanol, was purified to homogeneity and characterised. It was homodimeric with a subunit of Mr 48 000, and pI 3.6. It was optimally active at 45 °C and pH 9–10. It was fully stable at pH 6–7 for 24 h and 30 °C. Km values for d-xylitol and NAD+ were 94 mM and 0.14 mM, respectively. Mn2+ at 10 mM increased XDH activity 2-fold and Cu2+ at 10 mM inhibited activity completely.
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页码：2089 / 2092
页数：3
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