Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity

被引:10
|
作者
Chen, Siyu [1 ]
Sun, Xinyan [1 ]
Shi, Yajuan [1 ]
Wei, Ying [1 ]
Han, Xiaoyu [1 ]
Li, Honglian [1 ]
Chen, Linlin [1 ]
Sun, Bingjian [1 ]
Sun, Hangjun [1 ]
Shi, Yan [1 ]
机构
[1] Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Henan, Peoples R China
来源
VIRUSES-BASEL | 2019年 / 11卷 / 09期
基金
中国国家自然科学基金;
关键词
cucurbit chlorotic yellows virus; silencing suppressor; ubiquitin; SKP1; F-box; proteomics analysis; SCF UBIQUITIN LIGASE; 1ST REPORT; RNA; DEGRADATION; INSIGHTS; P0; ENCODES; RANGE;
D O I
10.3390/v11090818
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Plants use RNA silencing as a defense against viruses. In response, viruses encode various RNA silencing suppressors to counteract the antiviral silencing. Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a Cucumis sativus ortholog of S-phase kinase-associated protein 1 (SKP1). The F-box-like motif of p22 was identified through sequence analysis and found to be necessary for the interaction using a yeast two-hybrid assay. The involvement of the F-box-like motif in p22 silencing suppressor activity was determined. Proteomics analysis of Nicotiana benthamiana leaves expressing p22, and its F-box-like motif deletion mutant showed 228 differentially expressed proteins and five enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: ABC transporters, sesquiterpenoid and triterpenoid biosynthesis, ubiquitin-mediated proteolysis, riboflavin metabolism, and cysteine and methionine metabolism. Collectively, our results demonstrate the interaction between p22 and CsSKP1LB1 and show that the deletion of F-box-like motif inhibits p22 silencing suppressor activity. The possible pathways regulated by the p22 through the F-box-like motif were identified using proteomics analysis.
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页数:16
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