New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria

被引：755

作者：

Arnaud, M ^{[1
]}

Chastanet, A ^{[1
]}

Débarbouillé, M ^{[1
]}

机构：

[1] Inst Pasteur, Unite Biol Bacteries Pathogenes Gram Positif, CNRS, F-75724 Paris 15, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 11期

关键词：

D O I：

10.1128/AEM.70.11.6887-6891.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The plasmid was used in Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene.

引用

页码：6887 / 6891

页数：5

共 25 条

[1] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[2] INSERTIONAL MUTAGENESIS OF LISTERIA-MONOCYTOGENES WITH A NOVEL TN917 DERIVATIVE THAT ALLOWS DIRECT CLONING OF DNA FLANKING TRANSPOSON INSERTIONS [J].

CAMILLI, A ;

PORTNOY, DA ;

YOUNGMAN, P .

JOURNAL OF BACTERIOLOGY, 1990, 172 (07) :3738-3744

[3] THE PMTL NIC-CLONING VECTORS .1. IMPROVED PUC POLYLINKER REGIONS TO FACILITATE THE USE OF SONICATED DNA FOR NUCLEOTIDE SEQUENCING [J].

CHAMBERS, SP ;

PRIOR, SE ;

BARSTOW, DA ;

MINTON, NP .

GENE, 1988, 68 (01) :139-149

[4] Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria [J].

Chastanet, A ;

Fert, J ;

Msadek, T .

MOLECULAR MICROBIOLOGY, 2003, 47 (04) :1061-1073

[5] clpB, a novel member of the Listeria monocytogenes CtsR regulon, is involved in virulence but not in general stress tolerance [J].

Chastanet, A ;

Derre, I ;

Nair, S ;

Msadek, T .

JOURNAL OF BACTERIOLOGY, 2004, 186 (04) :1165-1174

[6]

FREES D, UNPUB

[7] CHARACTERIZATION OF STAPHYLOCOCCUS-AUREUS PLASMIDS INTRODUCED BY TRANSFORMATION INTO BACILLUS-SUBTILIS [J].

GRYCZAN, TJ ;

CONTENTE, S ;

DUBNAU, D .

JOURNAL OF BACTERIOLOGY, 1978, 134 (01) :318-329

[8] STRUCTURE OF A BETA-GALACTOSIDASE GENE OF BACILLUS-STEAROTHERMOPHILUS [J].

HIRATA, H ;

FUKAZAWA, T ;

NEGORO, S ;

OKADA, H .

JOURNAL OF BACTERIOLOGY, 1986, 166 (03) :722-727

[9]

Iordanescu S, 1976, Arch Roum Pathol Exp Microbiol, V35, P111

[10] THE TOXIC SHOCK SYNDROME EXOTOXIN STRUCTURAL GENE IS NOT DETECTABLY TRANSMITTED BY A PROPHAGE [J].

KREISWIRTH, BN ;

LOFDAHL, S ;

BETLEY, MJ ;

OREILLY, M ;

SCHLIEVERT, PM ;

BERGDOLL, MS ;

NOVICK, RP .

NATURE, 1983, 305 (5936) :709-712

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