Colorimetric and fluorometric monitoring of the helix composition of collagen-like peptides at the nM level

被引：15

作者：

Sun, Xiuxia ^{[1
]}

Fan, Jun ^{[1
]}

Li, Xuan ^{[1
]}

Zhang, Shanshan ^{[1
]}

Liu, Xiaoyan ^{[1
]}

Xiao, Jianxi ^{[1
]}

机构：

[1] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 15期

基金：

中国国家自然科学基金;

关键词：

DESIGN; HETEROTRIMERS; BETA;

D O I：

10.1039/c5cc09565d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have demonstrated that the incorporation of a dye-labeled collagen-like peptide in the homotrimeric versus heterotrimeric context results in visible color changes and distinct fluorescence. The unique fluorescence self-quenching assay can unambiguously determine the helix composition of heterotrimers at the nM level, far extending our capability to characterize a collagen triple helix.

引用

页码：3107 / 3110

页数：4

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