Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae

被引：415
|
作者：
Dean, Andrew P. [1 ]
Sigee, David C. [1 ]
Estrada, Beatriz [1 ]
Pittman, Jon K. [1 ]
机构：
[1] Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England
来源：
BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 12期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Microalgae; Nitrogen limitation; Lipid biosynthesis; Fourier transform infrared spectroscopy; Biofuel; ALGA NEOCHLORIS-OLEOABUNDANS; BIODIESEL PRODUCTION; NUTRIENT LIMITATION; CHLORELLA-VULGARIS; CARBON ALLOCATION; PHYTOPLANKTON; GROWTH; MICROSPECTROSCOPY; CELLS; BACILLARIOPHYCEAE;
D O I：
10.1016/j.biortech.2010.01.065
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In this study Fourier transform infrared micro-spectroscopy (FTIR) was used to determine lipid and carbohydrate content over time in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus subspicatus grown in batch culture in limiting concentrations of nitrogen (N). Both algae exhibited restricted cell division and increased cell size following N-limitation. FTIR spectra of cells in N-limited media showed increasing lipid:amide 1 and carbohydrate:amide 1 ratios over time. The use of lipid- and starch-staining dyes confirmed that the observed ratio changes were due to increased lipid and carbohydrate synthesis. These results demonstrate rapid metabolic responses of C. reinhardtii and S. subspicatus to changing nutrient availability, and indicate the efficiency of FIR as a reliable method for high-throughput determination of lipid induction. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4499 / 4507
页数：9
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