GENOME-WIDE MAPPING OF NUCLEOSOMES IN YEAST

被引：27
|
作者：
Rando, Oliver J. [1 ]
机构：
[1] Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA 01605 USA
来源：
METHODS IN ENZYMOLOGY, VOL 470: GUIDE TO YEAST GENETICS:: FUNCTIONAL GENOMICS, PROTEOMICS, AND OTHER SYSTEMS ANALYSIS, 2ND EDITION | 2010年 / 470卷
关键词：
POSITIONS; SEQUENCE; REGIONS;
D O I：
10.1016/S0076-6879(10)70005-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The packaging of eukaryotic genomes into chromatin has wide-ranging influences on all DNA-templated processes, from DNA repair to transcriptional regulation. The repeating subunit of chromatin is the nucleosome, which comprises 147 bp of DNA wrapped around an octamer of proteins. Positioning of nucleosomes relative to underlying DNA is a key factor in the regulation of gene transcription by chromatin, as DNA sequences between nucleosomes are more accessible to regulatory factors than are DNA sequences within nucleosomes. Here, I describe protocols for mapping nucleosome positions across the yeast genome.
引用
收藏
页码：105 / 118
页数：14
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