Highly selective NIR fluorescent probe for acetylcholinesterase and its application in pesticide residues detection

A series of near-infrared (NIR) fluorescent substrates (NDRO-1 similar to 8) derived from fluorophore NDRH with different volumes of ester bond as the recognition group were designed and synthesized for the detection of acetylcholinesterase (AChE), among which NDRO-1 with the smallest acetate group displayed the highest activity toward AChE. The detection limit of NDRO-1 for sensing AChE was 0.32 mu g/mL, and K-m was 6.40 mu mol/L, indicating ultra-sensitivity and good affinity of NDRO-1 toward AChE. NDRO-1 was used to detect the inhibitory of four kinds of pesticides including methamidophos, dichlorvos, and the detection limit was lower than 50 mu g/L, which was further used in pesticide residues detection. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.