Characterization of a Bacillus megaterium strain with metal bioremediation potential and in silico discovery of novel cadmium binding motifs in the regulator, CadC

被引:18
|
作者
Kumari, Weerasingha Mudiyanselage Nilmini Hasintha [1 ]
Thiruchittampalam, Shalini [2 ]
Weerasinghe, Mahinagoda Siril Samantha [2 ]
Chandrasekharan, Naduviladath Vishvanath [1 ]
Wijayarathna, Champika Dilrukshi [1 ]
机构
[1] Univ Colombo, Fac Sci, Dept Chem, Biotechnol Lab, Colombo 03, Sri Lanka
[2] Univ Colombo, Fac Sci, Dept Chem, Computat Chem Lab, Colombo 03, Sri Lanka
基金
美国国家科学基金会;
关键词
Metal binding motifs; Bacillus megaterium; CadC; CadA; Molecular dynamics simulation; Bioremediation potential; P-TYPE ATPASE; LEAD RESISTANCE; COPPER; SUBTILIS; GENE; COORDINATION; TRAFFICKING; DETERMINANT; MECHANISMS; PROTEIN;
D O I
10.1007/s00253-021-11193-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bioremediation of toxic metal ions using bacterial strains is a promising tool. Metal binding motifs in microbial proteins are involved in the regulation and transport of such toxic metals for metal detoxification. A bacterial strain designated TWSL_4 with metal (Cu, Cd, and Pb) resistance and removal ability was isolated and identified as a Bacillus megaterium strain using 16S rRNA gene analysis. An operon with 2 open reading frames (ORFs) was identified, cloned, and sequenced. ORF1 and ORF2 were identical to the cadmium efflux system accessory protein (CadC) and cadmium-translocating P-type ATPases (CadA) of B. megaterium strain YC4-R4 respectively. A protein homology search using Swiss model retrieved no crystal structures for CadC and CadA of Bacillus sp.. CadC of TWSL_4 had a sequence identity of 53% to the CadC (121aa) protein and 51.69% to the CadC crystal structure (1U2W.1.B; GMQE=0.75) of Staphylococcus sp. pI258. Molecular dynamic simulation studies revealed the presence of three metal binding regions in CadC of TWSL_4, [ASP7-TYR9], [ASP100-HIS102], and [LYS113-ASP116]. This is the first report showing evidence for the presence of Cd2+ and Zn2+ metal binding motifs in the CadC regulator of the Bacillus megaterium cad operon. The bacterial strain TWSL_4 was also found to contain two different P type ATPases encoding genes, cadA and zosA involved in metal resistance. Furthermore, the metal bioremediation potential of strain TWSL_4 was confirmed using an industrial effluent.
引用
收藏
页码:2573 / 2586
页数:14
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  • [1] Characterization of a Bacillus megaterium strain with metal bioremediation potential and in silico discovery of novel cadmium binding motifs in the regulator, CadC
    Weerasingha Mudiyanselage Nilmini Hasintha Kumari
    Shalini Thiruchittampalam
    Mahinagoda Siril Samantha Weerasinghe
    Naduviladath Vishvanath Chandrasekharan
    Champika Dilrukshi Wijayarathna
    Applied Microbiology and Biotechnology, 2021, 105 : 2573 - 2586