Breakthrough performance of stacks of dye-cellulosic fabric in affinity chromatography of lysozyme

被引：6

作者：

Tejeda-Mansir, A

Montesinos, RM

Magaña-Plaza, I

Guzmán, R

机构：

[1] IPN, CINVESTAV, Dept Biotecnol & Bioingn, Mexico City 07360, DF, Mexico

[2] Univ Sonora, Hermosillo 83000, Sonora, Mexico

[3] Univ Arizona, Dept Environm Chem & Engn, Tucson, AZ 85721 USA

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2003年 / 25卷 / 04期

关键词：

affinity chromatography; proteins; frontal analysis;

D O I：

10.1007/s00449-002-0302-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The breakthrough performance of stacks of dye-cellulosic fabric in affinity chromatography of lysozyme was investigated in batch and flow experiments. Breakthrough curves were significantly affected by flow rate and were not dependent on the feed solution concentration. System dispersion curves could not explain the flow-rate dependence. Breakthrough curves were analyzed by coupling the kinetic model for pore mass transfer as the only controlling resistance and a system dispersion model. From the analysis, pore film mass transfer resistance was found to be the leading rate-limiting factor when the residence time in the column is greater than 5 min. The model was used to predict the operating and design parameters needed to obtain sharp breakthrough curves. Selectivity studies using lysozyme and bovine serum albumin mixtures showed a high system selectivity for lysozyme.

引用

页码：235 / 242

页数：8

共 15 条

[1] Breakthrough performance of stacks of dye-cellulosic fabric in affinity chromatography of lysozyme
A. Tejeda-Mansir
R. Montesinos
I. Magaña-Plaza
R. Guzmán
Bioprocess and Biosystems Engineering, 2003, 25 : 235 - 242
[2] Direct lysozyme separation from egg white by dye membrane affinity chromatography
Grasselli, M
Camperi, SA
del Cañizo, AAN
Cascone, O
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1999, 79 (02) : 333 - 339
[3] Improved performance of column chromatography by arginine: Dye-affinity chromatography
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Ejima, Daisuke
Tsumoto, Kouhei
Ishibashi, Matsujiro
Tokunaga, Masao
PROTEIN EXPRESSION AND PURIFICATION, 2007, 52 (02) : 410 - 414
[4] Dye-Affinity Nanofibrous Membrane for Adsorption of Lysozyme: Preparation and Performance Evaluation
Wang, Steven Sheng-Shih
Yang, Szu-Ming
Hsin, Ai
Chang, Yu-Kaung
FOOD TECHNOLOGY AND BIOTECHNOLOGY, 2018, 56 (01) : 40 - 50
[5] Comparison of breakthrough performance using dye-affinity membrane disks and gel bead columns
Suen, SY
Tsai, YH
Chen, RL
SEPARATION SCIENCE AND TECHNOLOGY, 2000, 35 (04) : 573 - 591
[6] Affinity membrane chromatography:: relationship of dye-ligand type to surface polarity and their effect on lysozyme separation and purification
Arica, MY
Yilmaz, M
Yalçin, E
Bayramoglu, G
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 805 (02): : 315 - 323
[7] CHITIN-COATED CELITE AS AN AFFINITY ADSORBENT FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF LYSOZYME
YAMADA, H
FUKUMURA, T
ITO, Y
IMOTO, T
ANALYTICAL BIOCHEMISTRY, 1985, 146 (01) : 71 - 74
[8] High performance aptamer affinity chromatography for single-step selective extraction and screening of basic protein lysozyme
Han, Bin
Zhao, Chao
Yin, Junfa
Wang, Hailin
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2012, 903 : 112 - 117
[9] Performance of different metal-dye chelated affinity adsorbents of poly(2-hydroxyethyl methacrylate) in lysozyme separation
Arica, MY
Denizli, A
SEPARATION SCIENCE AND TECHNOLOGY, 2000, 35 (14) : 2243 - 2257
[10] Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification. II. Dynamic binding capacity using lysozyme as a model
Boto, R. E. F.
Anyanwu, U.
Sousa, F.
Almeida, R.
Queiroz, J. A.
BIOMEDICAL CHROMATOGRAPHY, 2009, 23 (09) : 987 - 993

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