Curious Effect of Isotope-Labelled Substrate/Co-Enzyme on Catalytic Activity of CO2 Reduction by Formate Dehydrogenase from Candida Boidinii

被引：5
|
作者：
Sato, Ryohei [1 ]
Amao, Yutaka [1 ,2 ]
机构：
[1] Osaka City Univ, Grad Sch Sci, Sumiyoshi Ku, 3-3-138 Sugimoto, Osaka 5588585, Japan
[2] Osaka City Univ, Res Ctr Artificial Photosynth ReCAP, Sumiyoshi Ku, 3-3-138 Sugimoto, Osaka 5588585, Japan
来源：
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN | 2022年 / 95卷 / 04期
关键词：
Biocatalytic CO2 reduction; Kinetic isotope effect; Formate dehydrogenase; CARBON-DIOXIDE; PHOTOSYNTHESIS;
D O I：
10.1246/bcsj.20220023
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The effects of isotope-labelled NADH and CO2 on formate dehydrogenase from Candida boidinii (CbFDH)-catalyzed CO2 reduction activity were investigated. It was found that the rate of CO2 reduction to formate with CbFDH decreased by using deuterium-labelled NADH (NADH-d(4)) as a co-enzyme. In contrast, the rate of CbFDH-catalyzed CO2 reduction to formate was unchanged in the use of (CO2)-C-13 as a substrate. In the substrate (CO2)-C-12 and (CO2)-C-13 is mixed, however, curious phenomenon on CbFDH-catalyzed CO2 reduction activity decrease was observed.
引用
收藏
页码：556 / 558
页数：3
相关论文
共 39 条
1234