Biological Significance of Hetero-Scaffolds Based Gold(III) Complexes

Synthesized ligands and complexes, [Au(L-n)Cl-2] Cl, have been characterized by various techniques such as elemental analysis, LC-MS, FT-IR, UV-Vis, H-1 and C-13 NMR spectroscopy, conductance measurement and magnetic moments measurement. The experimental results show that complexes exhibit higher antibacterial activity against Gram((+ve)) and Gram((-ve)) microorganisms than free ligands. The in vitro cytotoxicity and cellular level cytotoxicity suggest that Au(III) complexes show better activity than corresponding ligands. The DNA interaction study has been evaluated using absorption titration. The experimental evidence indicates (Kb = 1.08 - 3.44 . 105 M-1) that all the complexes have been bind to HS-DNA by intercalation mode. To further verify the nature of interaction viscosity measurement and molecular modeling have been carried out which suggest the intercalation binding between complex and DNA. The Schizosaccharomyces pombe cell DNA cleavage has been performed using agarose gel and their photographic images of complexes show smearing of DNA due to DNA cleavage from the nucleus.